Validating nutrient-related gene expression changes from microarrays using RT2 PCR-arrays

Genes & Nutrition

Table 1 Detectable reporters on RT² Profiler™ PCR-array in human PBMCs

Gene	Number of reporters on MA	FishOil versus Control		WeightLoss versus Control
Gene	Number of reporters on MA	FC–MA	FC–PCR	FC–MA	FC–PCR
ACTB	2	1.01	−1.05	1.03	−1.06
B2M	1	N/A	−1.09	N/A	−1.23*
BMP6	1	−1.02	−1.39	−1.17*	−1.15
BMP8B	1	N/A	−1.42	N/A	1.11
CSF1	4	1.11	1.04	−1.05	−1.15
FASLG	2	−1.01	−1.11	−1.04	−1.04
GAPDH	1	−1.01	−1.08	−1.07	−1.06
GDF11	1	−1.02	−1.09	−1.07	1.05
HPRT1	1	−1.00	−1.11	−1.11*	−1.05
IFNG	1	−1.03	−1.06	−1.21*	−1.07
IFNK	1	N/A	N/A	N/A	−1.38
IL15	1	−1.03	−1.21	−1.08	−1.05
IL16	1	1.03	−1.07	−1.13*	−1.08
IL18	1	−1.10	−1.13	1.02	−1.07
IL1B	1	1.05	−1.06	1.03	1.02
IL24	1	1.20*	−1.14	−1.29*	1.05
IL7	1	N/A	N/A	−1.19*	−1.43
LTA	1	1.01	1.05	−1.09*	−1.09
LTB	1	1.00	−1.05	−1.06*	−1.12
PDGFA	2	−1.10*	−1.24	−1.36*	−1.46
RPL13A	1	1.02	−1.01	−1.05	−1.08
TGFA	2	−1.03	1.03	−1.15*	−1.07
TGFB1	2	1.07	−1.03	−1.01	1.02
TGFB3	1	N/A	N/A	1.06	1.32
TNF	2	1.09*	−1.10	−1.07	−1.12
TNFSF10	1	−1.07*	−1.14	1.10*	−1.18
TNFSF12	1	1.03	−1.06	−1.03	1.03
TNFSF13	1	−1.02	−1.03	1.00	−1.01
TNFSF13B	1	−1.08	−1.23	−1.00	−1.03
TNFSF14	1	N/A	−1.33	N/A	1.02
TNFSF4	1	N/A	N/A	−1.28*	−1.48
TNFSF8	1	−1.08*	−1.25	1.01	1.02
TXLNA	1	−1.13*	1.08	1.07*	−1.06

Calculated fold changes of 34 detectable PCR gene products as compared to the fold changes derived from the microarray experiment for each intervention
FC fold change, MA microarray, N/A not available, PCR PCR-array
* P < 0.05

ISSN: 1865-3499