Table 2 Effect of LPS on gene expression level in PBMNC before and after vitamin C supplementation, analyzed using Human NFκB Signaling 96 STELLARRAY ™ qPCR array
Official gene symbol | Gene name | Baseline PBMNC | p valuea | Supplemented PBMNC | p valuea |
|---|---|---|---|---|---|
FC | FC | ||||
BCL3 | B-cell leukemia/lymphoma 3 | 2.08 ± 0.78 | 1 × 10−3 | 1.88 ± 0.51 | 5 × 10−4 |
EGR1 | Early growth response 1 | −3.01 ± 0.61 | 8 × 10−4 | −3.99 ± 0.88 | 9 × 10−6 |
EIF2AK2 | Eukaryotic translation initiation factor 2-alpha kinase 2 | 2.64 ± 0.89 | 3 × 10−3 | 2.84 ± 0.99 | 3 × 10−4 |
FOS | Proto-oncogene c-Fos | −4.52 ± 1.29 | 6 × 10−4 | −5.88 ± 0.80 | 1.5 × 10−7 |
IRAK2 | Interleukin-1 receptor-associated kinase 2 | 2.74 ± 1.04 | 3 × 10−3 | 2.77 ± 0.61 | 7 × 10−4 |
MAP2K6 | Mitogen-activated protein kinase kinase 6 | −3.33 ± 1.16 | 1 × 10−3 | −3.36 ± 1.18 | 1 × 10−4 |
MAP3K3 | Mitogen-activated protein kinase kinase kinase 3 | −1.35 ± 0.76 | 1 × 10−2 | −1.64 ± 0.57 | 5 × 10−4 |
MAPK11 | Mitogen-activated protein kinase 11 | 2.25 ± 0.58 | 6 × 10−4 | 2.40 ± 0.45 | 2 × 10−5 |
MAPK3 | Mitogen-activated protein kinase 3 | −1.14 ± 0.46 | 3 × 10−2 | −1.81 ± 0.41 | 6 × 10−3 |
NFKB1 | Nuclear factor NF-kappa-B p105 subunit | 2.64 ± 0.43 | 1 × 10−4 | 2.36 ± 0.17 | 3 × 10−6 |
NFKB1A | NF-kappa-B inhibitor alpha | 1.82 ± 0.62 | 6 × 10−3 | 1.07 ± 0.88 | 3 × 10−2 |
NFKB2 | Nuclear factor NF-kappa-B p100 subunit | 2.33 ± 0.18 | 1 × 10−5 | 2.02 ± 0.52 | 9 × 10−4 |
STAT1 | Signal transducer and activator of transcription 1-alpha/beta | 3.69 ± 0.86 | 4 × 10−5 | 3.82 ± 0.18 | 4 × 10−8 |
TIFA | TRAF-interacting protein with forkhead-associated domain | 1.97 ± 0.60 | 1 × 10−3 | 1.84 ± 0.53 | 1 × 10−5 |
TNFAIP3 | Tumor necrosis factor, alpha-induced protein 3 | 1.43 ± 0.55 | 4 × 10−3 | 1.30 ± 0.50 | 1 × 10−3 |
TNIP2 | TNFAIP3-interacting protein 2 | 1.05 ± 0.52 | 1 × 10−2 | 1.10 ± 0.35 | 8 × 10−3 |
TRAF3IP2 | Adapter protein CIKS | 2.04 ± 1.06 | 1 × 10−3 | 2.25 ± 0.57 | 2 × 10−3 |
IL1A | Interleukin-1 alpha | 6.10 ± 2.83 | 8 × 10−3 | 5.84 ± 1.68 | 1 × 10−3 |
IL1B | Interleukin-1 beta | 3.47 ± 1.61 | 8 × 10−3 | 3.16 ± 1.07 | 3 × 10−3 |
IL6 | Interleukin-6 | 9.24 ± 2.36 | 9 × 10−4 | 10.02 ± 1.48 | 1 × 10−4 |
IL8 | Interleukin-8 | 2.66 ± 1.70 | 2 × 10−2 | 2.43 ± 1.33 | 1 × 10−2 |
IL10 | Interleukin-10 | 3.30 ± 1.42 | 3 × 10−3 | 3.02 ± 0.96 | 1 × 10−3 |
CFLAR | CASP8 and FADD-like apoptosis regulator precursor | 1.05 ± 0.4 | 6 × 10−3 | 0.92 ± 0.34 | N.Sb |
MAP2K3 | Mitogen-activated protein kinase kinase 3 | 1.83 ± 0.81 | 2 × 10−3 | 0.97 ± 1.41 | N.S. |
MAP3K7IP1 | Mitogen-activated protein kinase kinase kinase 7-interacting protein 1 | 0.017 ± 0.60 | N.S. | −1.04 ± 0.45 | 1 × 10−2 |
MAP3K8 | Mitogen-activated protein kinase kinase kinase 8 | 2.89 ± 0.88 | 2 × 10−3 | 2.1 ± 2.27 | N.S. |
MAPK14 | Mitogen-activated protein kinase 14 | −0.82 ± 0.94 | N.S. | −1.19 ± 0.71 | 3 × 10−2 |
MYD88 | Myeloid differentiation primary response protein MyD88 | 1.12 ± 0.40 | 6 × 10−3 | 0.5 ± 0.46 | N.S. |
PPP1R13L | RelA-associated inhibitor | −0.49 ± 0.87 | N.S. | −1.18 ± 0.66 | 2 × 10−2 |
RELB | Transcription factor RelB | 2.68 ± 1.22 | 5 × 10−3 | 1.24 ± 0.53 | 7 × 10−4 |
TICAM1 | Toll-like receptor adaptor molecule 1 | 1.37 ± 0.59 | 2 × 10−2 | 0.94 ± 0.61 | N.S. |
TIRAP | Toll-interleukin 1 receptor (TIR) domain containing adaptor protein | −0.47 ± 0.21 | N.S. | −1.00 ± 0.41 | 7 × 10−3 |
TNFRSF11A | Tumor necrosis factor receptor superfamily member 11A precursor | −1.32 ± 1.13 | N.S. | −3.24 ± 0.82 | 3 × 10−4 |
TRADD | Tumor necrosis factor receptor type 1-associated DEATH domain protein | 1.51 ± 0.67 | 1 × 10−2 | 0.89 ± 0.32 | N.S. |
TNF-α | Tumor necrosis factor-alpha | 2.75 ± 0.83 | 2 × 10−3 | 1.26 ± 1.55 | N.S. |
ZAP70 | Tyrosine-protein kinase ZAP-70 | −0.23 ± 0.26 | N.S. | −1.11 ± 0.45 | 8 × 10−3 |