Table 1 List of primers used for quantitative real-time PCR
Gene | Primer name | Sequence (5′−3′) |
|---|---|---|
GAPDH | GAPDH F | AGTTCAACGGCACAGTCAAG |
GAPDH R | TACTCAGCACCAGCATCACC | |
PPARγ | PPARγ F | AAGACAACAGACAAATCACC |
PPARγ R | CAGGGATATTTTTGGCATACTC | |
SREBP1 | SREBP F | AAACCTGAAGTGGTAGAAAC |
SREBP R | TTATCCTCAAAGGCTGGG | |
NFKB | NFKB F | AAAAACGAGCCTAGAGATTG |
NFKB R | ACATCCTCTTCCTTGTCTTC | |
FAS | FAS F | AAAAGGAAAGTAGAGTGTGC |
FAS R | GACACATTCTGTTCACTACAG | |
ACSL | ACSL F | ACATTATGAACGATTGCTCC |
ACSL R | GCATTACACACTCTACAACG | |
MCAT | MCAT F | AAAACTCTAGGCTCAATCAAC |
MCAT R | GGATGTGTGTATTTATGCCC | |
TNFα | TNFα F | CTCACACTCAGATCATCTTC |
TNFα R | GAGAACCTGGGAGTAGATAAG |