Fig. 3

HiF diet Alb-NG37 mice developed dramatic phenotypes in comparison to HiF diet nTG, normal-diet Alb-NG37, and normal-diet nTG littermates. a Gross differences in body size are observed in HiF diet Alb-NG37 mice (TG#2) in comparison to normal-diet Alb-NG37 (TG#1), normal-diet nTG (nTG#1), and HiF diet nTG littermates (nTG#2). Weight increase quantified in panel d notes that body weight of HiF diet TG mice (n = 18) significantly exceeded that of normal-diet TG and nTG mice (n = 18 each, p < 0.01) and HiF diet nTG mice (n = 16, p < 0.05). HiF diet Alb-NG37 mice also exhibited liver enlargement (b) and cardiac enlargement (c) quantified as organ weight relative to body weight (e, f, respectively). e Liver weight of HiF diet TG mice (n = 18) significantly exceeded that of normal-diet TG and nTG mice (n = 18 each, p < 0.01) and HiF diet nTG mice (n = 16, p < 0.05). f Heart weight of HiF diet TG mice (n = 18) significantly exceeded that of normal-diet TG and nTG mice (n = 18 each, p < 0.01) and HiF diet nTG mice (in e, n = 16, p < 0.05). **p < 0.01; *p < 0.05