Table 1 List of genes considered on the basis of data interrogation and primers utilized in RT-PCR and PCR assay
From: Tocotrienols induce endoplasmic reticulum stress and apoptosis in cervical cancer cells
Gene | GenBank | Primers 5′→3′ | Size template |
|---|---|---|---|
β-actin (RT-PCR) | NM_001101.3 | F: AGAAGGATTCCTATGTGGGGG | 101 bp |
R: CATGTCGTCCCAGTTGGTGAC | |||
MIC-1 (RT-PCR) | NM_004864 | F: TGGTGCTCATTCAAAAGACCG | 123 bp |
R: GTGGAAGGACCAGGACTGCTC | |||
Cathepsin D (RT-PCR) | NM_001909 | F: CTGTGAGGCCATTGTGGACAC | 140 bp |
R: CAGCTTGTAGCCTTTGCCTCC | |||
XBP-1 (RT-PCR) | NM_005080.3 | F: GGAGTTAAGACAGCGCTTGGGGA | 118 bp |
R: TGTTCTGGAGGGGTGACAACTGGG | |||
CHOP (RT-PCR) | NM_001195057.1 | F: CCACACCTGAAAGCAGACTGATCCA | 102 bp |
R: TCATACCAGGCTTCCAGCTCCCA | |||
hXBP-1 (PCR) | NM_001079539.1 | F: AAACAGAGCAGCAGTCCAGACTGC | 472(u)bp 448(s)bp |
R: TCCTTCTGGGTAGACCTCTGGGAG |