Hepatic transcriptome implications for palm fruit juice deterrence of type 2 diabetes mellitus in young male Nile rats

Table 2 Genes selected for the real-time qRT-PCR validation experiment

Symbol	Definition	Accession	Assay ID
Target genes
Apoc1	Apolipoprotein C-I	NM_007469	Mm00431816_m1
Apoc3	Apolipoprotein C-III	NM_023114	Mm00445670_m1
Map3k11	Mitogen-activated protein kinase kinase kinase 11	NM_022012	Mm00491529_m1
Map3k2	Mitogen-activated protein kinase kinase kinase 2	NM_011946	Mm00442451_m1
Pik3r3	Phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 3 (p55)	NM_181585	Mm00725026_m1
Stxbp2	Syntaxin binding protein 2	NM_011503	Mm00441589_m1
Reference genes
Cct6a	Chaperonin containing Tcp1, subunit 6a (zeta)	NM_009838	Mm00486818_m1
Hpd	4-hydroxyphenylpyruvic acid dioxygenase	NM_008277	Mm00801734_m1
Nipbl	Nipped-B homologue (Drosophila)	NM_027707	Mm01297452_m1
Trim39	Tripartite motif-containing 39	NM_178281	Mm01273530_m1

The six target genes were selected based on their functional significance, their statistical significance, their presence as single splice transcripts in microarrays and their availability as Taqman assays designed across splice junctions. From the microarray data obtained, four candidate reference genes were also chosen to be tested for expression stability across the groups, with the three most stable ones being finally selected for relative quantification of the target genes

ISSN: 1865-3499