Fig. 5
From: Multiple effects of curcumin on promoting expression of the exon 7-containing SMN2 transcript
Curcumin-induced increase in SRSF1 is inhibited by trichostatin A (TSA). a Western blots of SRSF1 protein in the nuclei of the BJ301J cells upon 2 h of pretreatment with or without TSA, followed by 24-h addition of DMSO or curcumin. Nucleolin nuclear protein loading control. b Upper panel RT-PCR analysis of the SRSF1 transcripts in the cytoplasm of the BJ301J fibroblast cells upon 2 h of pretreatment with or without TSA, followed by 24-h addition of DMSO or curcumin. GAPDH RNA loading control. Lower panel A bar graph of the SRSF1 transcript changes (mean ± SD, n = 3) as in the upper panel. SRSF1 transcript was normalized to that of GAPDH and then to that of the sample treated with DMSO but not TSA (−), which is set as 100 %. **p < 0.01, ***p < 0.001, comparison between the indicated samples