Fig. 3

Effects of caffeic acid phenethyl ester (CAPE) on expression of matrix metalloproteinase (MMP)-2, MMP-3, and nuclear factor kappa B (NF-κB) DNA-binding activity in SK-Hep1 cells. a CAPE inhibits MMP-2 and MMP-9 expression in SK-Hep1 cells. Cells were treated with CAPE for 48 h. The activities of secreted MMP-2 (72 kDa) and MMP-9 (92 kDa) in the conditioned media were determined by gelatin zymography. b CAPE inhibits NF-κB DNA-binding activity in SK-Hep1 cells. SK-Hep1 cells were treated with CAPE for 1 h. The DNA-binding activity of NF-κB activation was determined by an electrophoretic mobility shift assay as described in “Materials and methods”, where CAPE was used at 12.5 and 25 μM. Data are representative of two independent experiments