Fig. 3
From: Resveratrol protects ROS-induced cell death by activating AMPK in H9c2 cardiac muscle cells
The effects of AMPK inhibition on the protective effect of resveratrol under H2O2 treatment. The H9c2 cells were infected with ad-wild type or ad-dominant negative AMPK constructs for 24 h, and then exposed to resveratrol for the indicated concentration, and then phosphorylation of AMPK was detected with western blot assay (upper). Under the same conditions, adenoviral-infected H9c2 cells were pretreated with resveratrol 50 μM for 30 min and then exposed to H2O2 500 μM for the indicated time periods. Cell death was measured by a MTT assay