Table 1 Primer sequences used for real-time PCR. GLUT4 Glucose transporter 4, IRS insulin receptor substrate, PPARγ peroxisome proliferators-activated receptor γ, C/EBPα CCAAT/enhancer-binding protein α, ADD1 adipocyte determination- and differentiation-dependent factor 1, aP2 adipocyte bindgin protein aP2, FAS fatty acid synthase, GAPDH glyceraldehyde-3-phosphate dehydrogenase
From: Soy pinitol acts partly as an insulin sensitizer or insulin mediator in 3T3-L1 preadipocytes
Gene | Forward primer | Reverse primer |
|---|---|---|
Adiponectin | ACGAGGGATGCTACTGTTGC | AAGCCCCCATACCAAATGT |
GLUT4 | GCCCCACAGAAGGTGATTGA | AGCGTAGTGAGGGTGCCTTG |
IRS | ATTGCTGGACAGTCTCCTC | CTTTTTCTTCACGAATGTCC |
PPARγ | AGAGTCTGCTGATCTGCGAGC | TTCCTGTCAAGATCGCCCTC |
C/EBPα | AAGAACAGCAACGAGTACC | AACTCCAGCACCTTCTGTT |
ADD1 | TGCCATGGGCAAGTACACAG | TTGCCATGGTATAGCATCTCCT |
aP2 | AGCATCATAACCCTAGATGG | AAACTCTTGTGGAAGTCACG |
FAS | GTGAAGAAGTGTCTGGACTGTGTCAT | TTTTCGCTCACGTGCAGTTTA |
GAPDH | TGCAGTGGCAAAGTGGAAT | TTGAATTTGCCGTGAGTGGA |