Effect of genistein on cholesterol metabolism-related genes in a colon cancer cell line

Genes & Nutrition

Table 2 Genistein effects on LDL receptor gene expression in DLD-1 cells

Genistein	30 min	1 h	2 h	5 h	24 h
Control	0.6 ± 0.03	0.62 ± 0.01	0.65 ± 0.02	0.63 ± 0.05	0.5 ± 0.04
0.01 μM	0.5 ± 0.02	0.6 ± 0.05	0.6 ± 0.05	1.2 ± 0.01*	0.9 ± 0.02*
1 μM	0.4 ± 0.02	0.5 ± 0.04	0.9 ± 0.03	1.1 ± 0.02*	1.1 ± 0.02*
10 μM	1.1 ± 0.06*	0.9 ± 0.02*	1.3 ± 0.07*	1.0 ± 0.02*	1.2 ± 0.06*
50 μM	1.5 ± 0.08*	1.2 ± 0.06*	1.4 ± 0.06*	1.2 ± 0.05*	1.0 ± 0.08*

Data displayed as mean ± SE of number molecules mRNA LDL receptor/number molecules mRNA β-actin. P value was determined by one-way analysis with Dunnett’s post-test. *P < 0.05 versus control. Results derived from triplicate cultures set up for each phytoestrogen concentration and for control, and each experiment was repeated four times

ISSN: 1865-3499