Effects of dietary carotenoids on mouse lung genomic profiles and their modulatory effects on short-term cigarette smoke exposures

Table 1 Primer sequences for real-time RT-PCR

Gene	Primer sequence (5′–3′)
GAPDH	Sense-GCAACAGGGTGGTG
GAPDH	Antisense-GGATAGGGCCTCTC
CYP1A1	Sense-CAGATGATAAGGTCATCACGA
CYP1A1	Antisense-TTGGGGATATAGAAGCCATTC
S100A8 (calgranulin A)	Sense-GCATCTCACAAAGACAGCCACA
S100A8 (calgranulin A)	Antisense-AGCCCTAGGCCAGAAGCTCT
S100A9 (calgranlin B)	Sense-GATGGCCAACAAAGCACCTT
S100A9 (calgranlin B)	Antisense-ATGATGGTGGTTATGCTGCG
Slfn4	Sense-ACAAGTGATGCCTGGAAAGG
Slfn4	Antisense-GACTGCCCTGGGAAATATGA
IL1-beta	Sense-CCAAAAGATGAAGGGCTGCT
IL1-beta	Antisense-TCATCTGGACAGCCCAGGTC
CBR-3	Sense-TACTTGGCTCTCCTGCCTCC
CBR-3	Antisense-GACTAGCTGGCCGTGAGGTT

The oligonucleotide sequence for each primer was obtained by Primer Express software using gene specific sequence from Affymetrix probe set data base. The primers were custom prepared and used as described in Sect. “Methods”

ISSN: 1865-3499