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Table 1 Nucleotide sequence of primers used in the RT-PCR assay

From: Chronic intracerebroventricular injection of TLQP-21 prevents high fat diet induced weight gain in fast weight-gaining mice

Gene

Forward primer

Reverse primer

Tα (°C)

No. of cycles

Amplicone (bp)

PPAR-δ

GTCGCACAACGCTATCCGCT

CCTTCTCTGCCTGCCACA

61

35 WAT

259

β1-AR

CGTCCGTCGTCTCCTTCTAC

TGATGATGCCCAGTGTCTTG

53

35 BAT

154

35 WAT

β2-AR

TGGTGGTGATGGTCTTTGTC

GTCTTGAGGGCTTTGTGCT

58

34 BAT

187

33 WAT

β3-AR

GCCGAGACTACAGACCATAACC

CGAGCATAGACGAAGAGCATT

56

34 BAT

483

30 WAT

UCP-1

AACAGAAGGATTGCCGAAACT

AATGAACACTGCCACACCTC

61

25 BAT

190

34 WAT

UCP-2

CAGTTCTACACCAAGGGCTCAGAG

TCTGTCATGAGGTTGGCTTTCAG

62

34 BAT

320

34 WAT

UCP-3

GGAGGAGAGAGGAAATACAGAGG

CCAAAGGCAGAGACAAAGTGA

58

28 BAT

218

33 WAT

HSL

GAGATTGAGGTGCTGTCG

TCGTGGGATTTAGAGGTC

55

26 WAT

350

GaPDH

GCCATCAACGACCCCTTCATTG

TGCCAGTGAGCTTCCCGTTC

53

27 BAT

603

33 WAT