Fig. 1

HSP-16.2 expression as visualized by GFP-fluorescence in a hsp-16.2p::GFP transgenic strain (CL2070). Green fluorescence of GFP in CL2070 exposed for 4 h to 100 μM juglone either on agar plates with E. coli as food source (left panel) or in axenic liquid culture medium (right panel) can be visualized from the fluorescence images as given in (a), left panels, and the overlays of fluorescence and light microscopy images (a, right panels). Time-dependent GFP-fluorescence is shown in (b) when CL2070 nematodes were exposed to 100 μM juglone in liquid axenic medium. Intensities of GFP expression in CL2070 cultured in liquid axenic medium for 4 h at various juglone concentrations is shown in (c). *P < 0.05, **P < 0.01, ***P < 0.001 versus the control