Fig. 2

Fisetin induces the expression of Nrf2, the ARE-specific transcription factor, and HO-1, an Nrf-2-dependent protein. Rat primary cortical neurons (7 days in culture) were untreated (ct) or treated with 1 or 5 μM fisetin for 1, 2 or 4 h (Nrf2) or with 1–10 μM fisetin for 24 h (HO-1). Nuclei were prepared (Nrf2) and equal amounts of protein were analyzed by SDS-PAGE and immunoblotting with Nrf2 antibodies or cell lysates were prepared and equal amounts of protein were analyzed by SDS-PAGE and immunoblotting with HO-1 antibodies. Immunoblotting with anti-actin is shown as a loading control. Similar results were obtained in three independent experiments