Fig. 1
From: Antioxidative defense and mitochondrial thermogenic response in brown adipose tissue
Time-dependent changes in Mn-SOD and CuZn-SOD mRNA amount in IBAT of untreated (Fig. 1a, d, respectively), l-arginine- (Fig. 1b, e, respectively) and l-NAME-treated (Fig. 1c, f, respectively) rats exposed to cold for different time-periods. Data obtained after quantification of Mn-SOD and CuZn-SOD mRNA bands by Quantity One® software (Software for Bio Rad’s Image Analysis system, Version 4.6.0). Expressions of transcripts were calculated as the ratio of intensities of Mn-SOD and CuZn-SOD genes and 18S rRNA house-keeping gene. Relative mRNA levels are presented as percentages of control acclimated to room temperature taken as 100%. The quantification data represents the mean±S.E.M. of three independent experiments. *Comparison with the control, * P < 0.05; ** P < 0.01; *** P < 0.001; #comparison of l-arginine- or l-NAME-treated group with untreated group in the same time point, # P < 0.05; ## P < 0.01; ### P < 0.001