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Fig. 2 | Genes & Nutrition

Fig. 2

From: Antioxidative defense and mitochondrial thermogenic response in brown adipose tissue

Fig. 2

Time course of changes in Mn-SOD and CuZn-SOD protein content in IBAT of untreated (Fig. 3a, d, respectively), l-arginine- (Fig. 3b, e, respectively) and l-NAME-treated (Fig. 3c, f, respectively) rats acclimated to low temperature. Western blotting was performed using specific antibody against Mn-SOD and CuZn-SOD. The results of a representative experiment from three observations are shown. Data obtained after quantification of Mn-SOD and CuZn-SOD protein bands by ImageQuant software. Relative protein concentrations are expressed in relation to the control acclimated to room temperature taken as 100%. Values represent the means±S.E.M. from three independent experiments. *Comparison with the control, *P < 0.05; **P < 0.01; ***P < 0.001; #comparison of L-arginine- or l-NAME-treated group with untreated group in the same time point, # P < 0.05; ## P < 0.01; ### P < 0.001. Volume is the sum of all the pixel intensities within a band; 1.0 pixel = 0.007744 mm2

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