Table 1 Primers sequences and cycling conditions
From: Antioxidative defense and mitochondrial thermogenic response in brown adipose tissue
Gene | Sequence | Product size (bp) | Cycle number |
|---|---|---|---|
MnSOD | |||
Forward | 5′-CGCAGCTAGGAATAATGGAA-3′ | 649 | 33 |
Reverse | 5′-TTATGACCCGCACTTACTGG-3′ | ||
Cycle protocol | 60″ at 95°C, 60″ at 57°C, 90″ at 72°C | ||
CuZnSOD | |||
Forward | 5′-TGGGGACAATACACAAGG-3′ | 359 | 33 |
Reverse | 5′-TAGCAGGACAGCAGATGA-3′ | ||
Cycle protocol | 60″ at 95°C, 60″ at 53°C, 90″ at 72°C | ||
18s rRNA | |||
Forward | 5′-CGCAGCTAGGAATAATGGAA-3′ | 860 | 33 |
Reverse | 5′-TTATGACCCGCACTTACTGG-3′ | ||
Cycle protocol | 30″ at 95°C, 30″ at 56°C, 30″ at 72°C | ||