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Table 6 Microarray vs. real-time quantitative RT-PCR (qRT-PCR) for selected genes in liver and adipose tissue (fat) from tabw2 and control mice

From: Gene expression profiles of a mouse congenic strain carrying an obesity susceptibility QTL under obesigenic diets

 

Microarray

qRT-PCR

Probe set ID

Symbol

Gene name

Tissue

Fold

Fold (CI)

1416946_a_at

Acaala

Acetyl-Coenzyme A acyltransferase 1A

Liver

−1.32

−1.08 (−3.08, 2.60)

1434185_at

Acaca

Acetyl-Coenzyme A carboxylase alpha

Fat

2.03

3.21 (−1.19, 12.34)

1422479_at

Acss2

Acyl-CoA synthetase short-chain family member 2

Fat

4.46

2.35 (1.19, 4.66)

1426454_at

Arhgdib

Rho, GDP dissociation inhibitor (GDI) beta

Liver

1.58

1.42 (−2.60, 4.21)

   

Fat

 

1.43 (1.05, 2.17)

1448229_s_at

Ccnd2

Cyclin D2

Liver

−1.69

−1.71 (−9.33, 3.18)

1434745_at

  

Fat

4.67

1.04 (−1.40, 1.52)

1423257_at

Cyp4a14

Cytochrome P450, family 4, subfamily a, polypeptide 14

Liver

−3.75

−1.15 (−61.22, 46.19)

1431035_at

Daaml

Dishevelled associated activator of morphogenesis 1

Fat

−1.38

−1.27 (−1.80, 1.10)

1436756_x_at

Hadhsc

L-3-hydroxyacyl-Coenzyme A dehydrogenase, short chain

Liver

−1.54

−1.36 (−2.06, 1.11)

1455777_x_at

Hsd17b4

Hydroxysteroid (17-beta) dehydrogenase 4

Liver

−1.29

−1.06 (−2.04, 1.80)

1460245_at

Klrdl

Killer cell lectin-like receptor, subfamily D, member 1

Fat

3.39

6.22 (2.97, 13.03)

1451022_at

Lrp6

Low density lipoprotein receptor-related protein 6

Liver

−1.29

−1.06 (−2.60, 2.31)

   

Fat

−1.92

1.70 (−1.09, 3.19)

1442560_at

Mgll

Monoglyceride lipase

Liver

−2.01

−3.63 (−14.2, 1.07)

1434110_x_at

Mup1

Major urinary protein 1

Fat

7.74

5.44 (2.40, 12.29)

1419976_s_at

Nfatc3

Nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3

Liver

−1.50

−1.05 (−2.89, 2.62)

   

Fat

−1.52

−1.03 (−1.30, 1.21)

1419112_at

Nlk

Nemo like kinase

Liver

−1.53

−1.13 (−1.50, 1.15)

1436359_at

Ret

Ret proto-oncogene

Fat

−3.46

−4.16 (−13.91, −1.24)

1416585_at

Ruvbl1

RuvB-like protein 1

Liver

−1.30

1.59 (−1.16, 2.97)

1436075_at

Sfrp5

Secreted frizzled-related sequence protein 5

Fat

−8.59

−3.98 (−13.07, −1.21)

1450117_at

Tcf3

Transcription factor 3

Fat

−1.64

1.04 (−2.05, 2.23)

1429428_at

Tcf7l2

Transcription factor 7-like 2, T-cell specific, HMG-box

Liver

−1.33

−1.59 (−3.39, 1.33)

1422602_a_at

Wnt5b

Wingless-related MMTV integration site 5B

Liver

1.19

1.01 (−3.63, 3.75)

   

Fat

 

2.56 (1.04, 6.85)

  1. ‘−’ indicates up-regulation and ‘no sign’ indicates down-regulation in tabw2 mice compared to control mice; CI=95%, confidence interval (lower limit, upper limit)