Table 1 Primer sequences (5′ → 3′)
From: Nrf2 target genes are induced under marginal selenium-deficiency
Gene name | Acc. number | Primer sequence | Product (bp) | TA (°C) |
|---|---|---|---|---|
Gclc | NM_010295.1 | fwd CCGCTGTCCAAGGTTGACGA rev GTTGCCGCCTTTGCAGATGTC | 101 | 60 |
Gpx2 | NM_030677 | fwd GTGCTGATTGAGAATGTGGC rev AGGATGCTCGTTCTGCCCA | 252 | 60 |
Gsta1/a2 | NM_008181.2 | fwd GCAGACCAGAGCCATTCTCAACT rev CAAGGTAGTCTTGTCCATGGCTC | 247 | 60 |
Gstm1 | NM_010358.4 | fwd AGCTCATCATGCTCTGTTACAACC rev AATCCACATAGGTGACCTTGTCCC | 143 | 62 |
Gstp1 | NM_013541.1 | fwd CAAATATGTCACCCTCATCTACACCA rev CAAAGGAGATCTGGTCACCCAC | 148 | 62 |
Hmox1 | NM_010442.1 | fwd CCTGGTGCAAGATACTGCCC rev GAAGCTGAGAGTGAGGACCCA | 105 | 60 |
Mt2 | NM_008630.2 | fwd CTGTGCCTCCGATGGATCCT rev CTTGTCGGAAGCCTCTTTGCAG | 150 | 62 |
Nqo1 | NM_008706.4 | fwd ATGTACGACAACGGTCCTTTCCAG rev GATGCCACTCTGAATCGGCCA | 134 | 60 |
Prdx1 | NM_011034.3 | fwd CAAGTGATTGGCGCTTCTGTGG rev AATGGTGCGCTTGGGATCTG | 122 | 62 |
Sepw1 | NM_009156.2 | fwd ATGCCTGGACATTTGTGGCGA rev GCAGCTTTGATGGCGGTCAC | 152 | 60 |
Sod1 | NM_011434.1 | fwd GGACAATACACAAGGCTGTACC rev CAGTCACATTGCCCAGGTCTC | 112 | 60 |
Srxn1 | NM_029688.4 | fwd AGCCTGGTGGACACGATCCT rev TGCTGGTAGGCTGCATAGCG | 130 | 62 |
Sult1b1 | NM_019878.3 | fwd CTGCCCACAGCAATGATGGA rev GCATCAAATTGCTCAGTTTGGGTC | 106 | 62 |
Txnrd1 | NM_015762.1 | fwd TACTGCATCAGCAGTGATGATC rev CCATGTTCTTCCATGTGTTCAC | 206 | 60 |
Ugt1a6a/b | NM_201410 | fwd CACGTGCTACCTAGAGGCACAG rev GACCACCAGCAGCTTGTCAC | 142 | 62 |
Reference genes | ||||
Hprt1 | NM_013556 | fwd GCAGTCCCAGCGTCGTG rev GGCCTCCCATCTCCTTCAT | 168 | 60 |
Rpl13a | NM_009438 | fwd GTTCGGCTGAAGCCTACCAG rev TTCCGTAACCTCAAGATCTGCT | 157 | 60 |