Table 1 Primer sequences for the selected genes used for validation of the microarray data using semi-quantitative RT–PCR and real-time RT–PCR
Gene | Primer sequence | Product size (bp) |
|---|---|---|
Glyceraldehyde-3-phosphate dehydrogenase (GADPH) (NM_002046) | Forward: 5′-TGCCTCCTGCACCACCAACTGC-3′ Reverse: 5′-AATGCCAGCCCCAGCGTCAAAG-3′ | 457 |
Phosphodiesterase 3A (PDE3A) (NM_000921) | Forward: 5′-GAACAGATGACACTGCTCAAGTT-3′ Reverse: 5′-GAGCAAGAATTGGTTTGTCCAG-3′ | 180 |
Interferon-induced protein with tetratricopeptide repeats (IFIT1) (NM_001548) | Forward: 5′-AGAACGGCTGCCTAATTTACAG-3′ Reverse: 5′-GCTCCAGACTATCCTTGACCTG-3′ | 73 |
Glutathione S-transferase A1 (GSTA1) (NM_145740) | Forward: 5′-AGCCGGGCTGACATTCATCT-3′ Reverse: 5′-TGGCCTCCATGACTGCGTTA-3′ | 230 |
Microsomal triglyceride transfer protein (MTTP) (NM_000253) | Forward: 5′-CGTTCGGCATCTACTTACAGC-3′ Reverse: 5′-GTTCTCCTCCCCCTCGTCAG-3′ | 180 |