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Table 1 Genes investigated

From: Gene regulation in β-sitosterol-mediated stimulation of adipogenesis, glucose uptake, and lipid mobilization in rat primary adipocytes

Gene name and abbreviation

Assay ID

Accession number

Protein kinase B (Akt)

Rn00583646_m1

NM_033230.1

Glucose transporter 4 (GLUT4)

Rn00562597_m1

NM_012751.1

Hormone sensitive lipase (HSL)

Rn00689222_m1

NM_012859.1

Phosphatidylinositol-3-kinase (PI3 K)

Rn00564547_m1

NM_013005.1

  1. General abbreviation of genes selected for this study and corresponding assay ID and accession information are available on Applied Biosystems website (www.appliedbiosystems.com). Assay ID refers to the “Applied Biosystems Gene Expression Assays” inventoried kit with proprietary primer and TaqMan® probe mix. Assay ID with “Rn” prefix denotes “Rattus norvegicus”. All gene expression assay kits used were FAM/MGB probed. Eukaryotic 18S rRNA was used as the endogenous control