Genetic polymorphisms influence runners’ responses to the dietary ingestion of antioxidant supplementation based on pequi oil (Caryocar brasiliense Camb.): a before-after study

Miranda-Vilela, Ana Luisa; Lordelo, Graciana Souza; Akimoto, Arthur Kenji; Alves, Penha Cristina Zaidan; Pereira, Luiz Carlos da Silva; Klautau-Guimarães, Maria de Nazaré; Grisolia, Cesar Koppe

doi:10.1007/s12263-011-0217-y

Table 7 Influences of Haptoglobin (Hp), MnSOD, CAT, GPx-1, ACE, GSTM1, GSTT1, CK NcoI, CK TaqI, CRP G1059C, MTHFR C677T, and MTHFR A1298C genes’ polymorphisms on the CRP, hs-CRP, arterial pressure and TBARS assay before and after pequi-oil supplementation

From: Genetic polymorphisms influence runners’ responses to the dietary ingestion of antioxidant supplementation based on pequi oil (Caryocar brasiliense Camb.): a before-after study

Genetic Markers	CRP (mg/dL)		hs-CRP (mg/dL)		Systolic pressure (mmHg)		Diastolic pressure (mmHg)		TBARS (nmol/mL of MDA)
Genetic Markers	Before	After	Before	After	Before	After	Before	After	Before	After
Hp
1F-1F	0.24 ± 0.07	0.31 ± 0.11	1.02 ± 0.27	1.41 ± 0.53	111.43 ± 4.59	112.86 ± 2.86	68.57 ± 4.04	65.71 ± 2.97	0.029 ± 0.003	0.030 ± 0.002
1F-1S	0.28 ± 0.12	0.35 ± 0.09	1.97 ± 0.80	1.39 ± 0.39	120.00 ± 1.89	115.00 ± 1.89*^,≠	77.50 ± 3.13	72.50 ± 2.50	0.032 ± 0.002	0.030 ± 0.003
1S-1S	0.30 ± 0.05	0.37 ± 0.07	1.49 ± 0.51	1.95 ± 0.43	116.00 ± 1.97	111.00 ± 1.76*^,≠	73.50 ± 1.67	69.00 ± 1.76	0.023 ± 0.001*,^b	0.025 ± 0.001
1F-2	0.32 ± 0.08	0.38 ± 0.07	1.70 ± 0.50	2.00 ± 0.65	115.26 ± 1.77	113.16 ± 2.17	71.05 ± 2.28	70.00 ± 1.87	0.028 ± 0.002	0.026 ± 0.002
1S-2	0.36 ± 0.06	0.36 ± 0.05	1.37 ± 0.38	1.34 ± 0.33	116.88 ± 1.23	111.87 ± 1.76*^,≠	72.19 ± 1.54	68.12 ± 1.45	0.029 ± 0.001	0.026 ± 0.001
2-2	0.36 ± 0.07	0.38 ± 0.06	1.82 ± 0.46	1.36 ± 0.24	112.50 ± 1.00	111.87 ± 1.14	73.13 ± 1.52	69.69 ± 1.52	0.024 ± 0.001	0.025 ± 0.001
P values	0.827	0.969	0.912	0.604	0.060	0.680	0.358	0.599	0.005	0.249
MnSOD
Val/Val	0.34 ± 0.06	0.33 ± 0.04	1.38 ± 0.37	1.62 ± 0.31	116.50 ± 1.67	110.50 ± 1.85*^,≠	71.00 ± 2.16	68.50 ± 1.82	0.031 ± 0.002	0.028 ± 0.002
Val/Ala	0.33 ± 0.04	0.37 ± 0.03	1.59 ± 0.24	1.54 ± 0.20	114.95 ± 0.79	112.69 ± 0.85*^,≠	73.01 ± 0.89	69.35 ± 0.84*^,≠	0.026 ± 0.001*,^a	0.026 ± 0.001
Ala/Ala	0.30 ± 0.11	0.60 ± 0.31	2.58 ± 0.99	1.51 ± 0.52	114.00 ± 4.00	110.00 ± 3.16	72.00 ± 4.90	68.00 ± 4.90	0.026 ± 0.003	0.026 ± 0.003
P values	0.885	0.872	0.398	0.478	0.786	0.501	0.575	0.853	0.024	0.493
CAT
AA	0.33 ± 0.05	0.42 ± 0.08	1.97 ± 0.54	2.10 ± 0.60	115.22 ± 1.65	110.87 ± 1.39*^,≠	70.87 ± 1.65	69.13 ± 1.65	0.027 ± 0.001	0.027 ± 0.001
AT	0.35 ± 0.05	0.39 ± 0.05	1.39 ± 0.30	1.31 ± 0.21	114.58 ± 1.07	111.46 ± 1.33*^,≠	73.75 ± 1.32	69.79 ± 1.21*^,≠	0.026 ± 0.001	0.025 ± 0.001
TT	0.31 ± 0.05	0.33 ± 0.03	1.61 ± 0.33	1.56 ± 0.23	115.74 ± 1.13	113.62 ± 1.11	72.34 ± 1.30	68.51 ± 1.18*^,≠	0.027 ± 0.001	0.028 ± 0.001
P values	0.877	0.735	0.344	0.262	0.628	0.212	0.332	0.740	0.837	0.133
GPx-1
Pro/Pro	0.36 ± 0.05	0.34 ± 0.04	2.12 ± 0.38	1.57 ± 0.23	115.69 ± 0.99	112.76 ± 1.15*^,≠	73.97 ± 1.04	68.97 ± 1.03*^,≠	0.027 ± 0.001	0.026 ± 0.001
Pro/Leu	0.29 ± 0.03	0.38 ± 0.04	1.08 ± 0.17	1.69 ± 0.31	115.53 ± 1.13	111.06 ± 1.02**^,≠	71.28 ± 1.35	68.94 ± 1.23*^,≠	0.027 ± 0.001	0.026 ± 0.001
Leu/Leu	0.32 ± 0.06	0.45 ± 0.12	1.04 ± 0.22	0.97 ± 0.22	111.54 ± 1.91	113.85 ± 2.67	71.54 ± 2.96	70.77 ± 2.39	0.027 ± 0.002	0.026 ± 0.002
P values	0.851	0.542	0.118	0.507	0.227	0.377	0.204	0.726	0.998	0.912
ACE
DD	0.33 ± 0.06	0.39 ± 0.05	1.72 ± 0.42	1.57 ± 0.28	113.33 ± 1.06	111.54 ± 1.01	71.03 ± 1.41	68.46 ± 1.25	0.027 ± 0.001	0.026 ± 0.001
ID	0.33 ± 0.04	0.34 ± 0.03	1.55 ± 0.27	1.55 ± 0.25	115.67 ± 1.02	112.83 ± 1.01*^,≠	72.67 ± 1.11	70.00 ± 1.01	0.027 ± 0.001	0.027 ± 0.001
II	0.34 ± 0.05	0.43 ± 0.08	1.41 ± 0.33	1.49 ± 0.41	117.37 ± 1.85	111.58 ± 2.79*^,≠	75.79 ± 2.07	67.89 ± 2.24**^,≠	0.026 ± 0.002	0.026 ± 0.001
P values	0.590	0.653	0.993	0.922	0.141	0.448	0.206	0.407	0.840	0.639
GSTM1
Null	0.36 ± 0.04	0.36 ± 0.03	1.83 ± 0.30	1.54 ± 0.19	115.26 ± 0.93	112.50 ± 0.90*^,≠	72.63 ± 1.05	69.61 ± 0.98*^,≠	0.027 ± 0.001	0.027 ± 0.001
Non-null	0.27 ± 0.03	0.38 ± 0.05	1.12 ± 0.18	1.57 ± 0.33	115.00 ± 1.04	111.67 ± 1.36*^,≠	72.62 ± 1.28	68.33 ± 1.13*^,≠	0.025 ± 0.001	0.026 ± 0.001
P values	0.443	0.902	0.513	0.652	0.978	0.674	0.915	0.503	0.183	0.436
GSTT1
Null	0.33 ± 0.06	0.27 ± 0.06	1.68 ± 0.47	1.30 ± 0.44	114.71 ± 2.12	111.76 ± 2.14	70.00 ± 2.10	68.82 ± 2.08	0.029 ± 0.001	0.027 ± 0.002
Non-null	0.33 ± 0.03	0.38 ± 0.03	1.57 ± 0.23	1.59 ± 0.18	115.25 ± 0.74	112.28 ± 0.81**^,≠	73.07 ± 0.88	69.21 ± 0.80**^,≠	0.026 ± 0.001	0.026 ± 0.001
P values	0.665	0.083	0.409	0.492	0.825	0.851	0.216	0.864	0.152	0.791
CK NcoI
AA	0.35 ± 0.05	0.38 ± 0.04	1.63 ± 0.33	1.74 ± 0.27	115.25 ± 0.92	111.97 ± 0.87*^,≠	73.11 ± 1.11	69.02 ± 1.01**^,≠	0.026 ± 0.001	0.027 ± 0.001
AG	0.32 ± 0.04	0.36 ± 0.04	1.63 ± 0.29	1.47 ± 0.23	115.10 ± 1.10	111.76 ± 1.33*^,≠	71.76 ± 1.24	69.02 ± 1.20	0.028 ± 0.001	0.026 ± 0.001
GG	0.25 ± 0.09	0.33 ± 0.05	0.85 ± 0.30	0.59 ± 0.12	115.00 ± 4.28	118.33 ± 3.07	75.00 ± 4.28	71.67 ± 3.07	0.026 ± 0.003	0.026 ± 0.002
P values	0.836	0.939	0.737	0.287	0.976	0.157	0.635	0.682	0.276	0.780
CK TaqI
1-1	0.37 ± 0.08	0.43 ± 0.08	1.60 ± 0.49	2.11 ± 0.50	117.27 ± 2.37	110.91 ± 2.11*^,≠	77.27 ± 2.73	72.73 ± 2.37	0.026 ± 0.002	0.028 ± 0.002
1-2	0.30 ± 0.04	0.40 ± 0.05	1.29 ± 0.24	1.71 ± 0.30	115.00 ± 0.93	111.90 ± 1.06*^,≠	71.55 ± 1.04	67.93 ± 1.07*^,≠	0.026 ± 0.001	0.026 ± 0.001
2-2	0.35 ± 0.06	0.32 ± 0.02	1.91 ± 0.39	1.26 ± 0.16	114.90 ± 1.17	112.86 ± 1.24	72.86 ± 1.37	69.80 ± 1.15	0.028 ± 0.001	0.027 ± 0.001
P values	0.828	0.625	0.362	0.157	0.701	0.617	0.161	0.128	0.341	0.638
CRP G1059C
GG	0.34 ± 0.03	0.37 ± 0.03	1.67 ± 0.23	1.60 ± 0.18	115.14 ± 0.73	112.19 ± 0.83**^,≠	72.48 ± 0.85	69.43 ± 0.78**^,≠	0.027 ± 0.001	0.026 ± 0.001
GC	0.27 ± 0.06	0.35 ± 0.08	0.86 ± 0.25	1.14 ± 0.45	115.38 ± 2.43	112.31 ± 1.22	73.85 ± 2.67	66.92 ± 2.63	0.025 ± 0.002	0.026 ± 0.002
P values	0.645	0.785	0.144	0.103	0.974	0.996	0.568	0.197	0.349	0.666
MTHFR C677T
CC	0.28 ± 0.05	0.36 ± 0.04	1.30 ± 0.29	1.49 ± 0.24	115.18 ± 1.05	111.43 ± 1.12**^,≠	72.50 ± 1.20	68.93 ± 0.94*^,≠	0.028 ± 0.001	0.027 ± 0.001
CT	0.36 ± 0.05	0.39 ± 0.05	1.76 ± 0.33	1.74 ± 0.31	115.00 ± 1.02	112.61 ± 1.22	72.39 ± 1.21	68.48 ± 1.20*^,≠	0.025 ± 0.001	0.026 ± 0.001
TT	0.39 ± 0.05*,^a	0.33 ± 0.04	1.99 ± 0.61	1.19 ± 0.24	115.63 ± 2.23	113.75 ± 1.80	73.75 ± 2.56	71.88 ± 2.77	0.026 ± 0.002	0.023 ± 0.002
P values	0.063	0.992	0.648	0.944	0.899	0.670	0.879	0.557	0.133	0.079
MTHFR A1298C
AA	0.38 ± 0.04	0.38 ± 0.04	1.65 ± 0.26	1.49 ± 0.22	115.35 ± 0.82	112.68 ± 0.98*^,≠	72.82 ± 1.05	68.73 ± 0.96**^,≠	0.027 ± 0.001	0.026 ± 0.001
AC	0.25 ± 0.04**,^a	0.35 ± 0.04*	1.51 ± 0.36	1.71 ± 0.28	114.67 ± 1.30	111.33 ± 1.21*^,≠	72.22 ± 1.34	69.56 ± 1.23	0.026 ± 0.001	0.027 ± 0.001
CC	0.34 ± 0.16	0.33 ± 0.18	0.67 ± 0.07	0.56 ± 0.06	120.00 ± 0.00	115.00 ± 5.00	75.00 ± 5.00	75.00 ± 5.00	0.036 ± 0.001	0.034 ± 0.004
P values	0.006	0.760	0.397	0.727	0.511	0.692	0.894	0.459	0.123	0.208

Data are expressed as mean ± SEM (standard error of mean). MDA = malondialdehyde. For GSTM1, GSTT1 and CRP G1059C, P values were generated by the independent samples t-Test (Tbars assay) or Mann–Whitney U test (other variables); for the other genetic markers, P values were generated by ANOVA (Tbars assay) or by the Kruskall–Wallis test (other variables). Asterisks indicate significant (* P < 0.05) and highly significant (** P < 0.01) differences. The lower case letters indicate significant differences between two specific genotypes, with a = significant compared with the first genotype of each genetic marker; b = significant compared with the second genotype of each genetic marker; c = significant compared with the third genotype of each genetic marker. For Hp polymorphism, the lower case letters d, e, and f were also added, with d = significant compared with 1F-2; e = significant compared with 1S-2; and f = significant compared with 2-2 genotypes. The symbol ≠ indicates significant differences in the comparison of before-after pequi-oil supplementation detected by the Wilcoxon matched pairs test

Back to article page

ISSN: 1865-3499

Contact us

General enquiries: journalsubmissions@springernature.com

Genes & Nutrition

Contact us