Fig. 1

Multiplex PCR—Expression of Angiogenesis and related genes in mouse tumour samples. Total RNA was isolated from the samples (a). After reverse transcription, a multiplex polymerase chain reaction with primers for the three genes SET was performed. After 40 cycles of amplification, the samples were electrophoresed and stained with ethidium bromide. P Positive size marker, Lane 1: C, Lane 2: E (c, d, e). cDNA Normalization using House Keeping Gene Panels [SDHA succinate dehydrogenase complex, subunit A, HPRT1 hypoxanthine guanine phosphoribosyltransferase 1, B2M beta-2-microglobulin] (b)