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Table 2 Overview of human studies that demonstrate an association between IBS and compositional dysbiosis of the intestinal microbiota determined with culture-independent methods

From: Intestinal microbiota in human health and disease: the impact of probiotics

Study material Population Analytical methods References
Faeces (3 time points) 27 IBS patients
22 Healthy individuals
qPCR Malinen et al. (2005)*
Faeces (3 time points) 26 IBS patients
25 Healthy individuals
Conventional culturing
DGGE
Clone library sequencing (16S)
Mättö et al. (2005)*
Biopsies: inflamed and non-inflamed tissue (ileum, ascending/sigmoid colon) 20 CD patients
20 UC patients
20 Self-limiting colitis patients
20 IBS patients
20 Healthy individuals
FISH Swidsinski et al. (2005)
Faeces (2 time points) 16 IBS patients
16 Healthy individuals
DGGE
TRAC
Maukonen et al. (2006)*
Faeces 24 IBS patients
23 Healthy individuals
G+C based profiling
Clone library sequencing (16S)
qPCR
Kassinen et al. (2007)*
Duodenal biopsies
Faeces
41 IBS patients
26 Healthy individuals
FISH
qPCR
Kerckhoffs et al. (2009)#
Faeces 10 (+2) IBS (only IBS-D)
23 Healthy individuals
G+C based profiling
Clone library sequencing (16S)
qPCR
Krogius–Kurikka et al. (2009)*
Faeces (3 time points) 20 IBS patients
15 Healthy individuals
qPCR Lyra et al. (2009)*
Colonic biopsies
Faeces
10 IBS patients (only IBS-D)
10 Healthy individuals
Conventional culturing
qPCR
Carroll et al. (2010)
Colonic biopsies
Faeces
47 IBS patients
33 Healthy individuals
DGGE Codling et al. (2010)
Duodenal biopsies
Faeces
37 IBS patients
20 Healthy individuals
DGGE
Clone library sequencing (16S)
qPCR
Kerckhoffs et al. (2010)#
Faeces 44 IBS patients qPCR Malinen et al. (2010)*
Faeces 26 IBS patients
26 Healthy individuals
Conventional culturing
qPCR
HPLC
Tana et al. (2010)
  1. All studies have applied Rome II or III criteria to recruit their subjects and categorise them in IBS subtypes. Studies that have used subjects from the same cohort are indicated by * and #
  2. DGGE denaturing gradient gel electrophoresis, FISH fluorescence in situ hybridisation, HPLC high-performance liquid chromatography, qPCR quantitative polymerase chain reaction, TRAC transcript analysis with the aid of affinity capture