Fig. 1

Flow cytometric characterization of resveratrol-induced HL1-NB cell death. Cell death induced by resveratrol (Rsv) on tumoral cardiac mouse HL1-NB cells was determined by various flow cytometric methods, a: Dead cells were characterized with propidium iodide (PI) staining, as it enters dead cells only, b: Hoechst 33342 staining was used to distinguish between normal and apoptotic cells (normal cells have round and regular nuclei whereas apoptotic cells have condensed and/or fragmented nuclei), c: PI was also used to analyze the proportion of the cells in different phases of the cell cycle (The subG1 peak contains only apoptotic cells). Data are representative of three independent experiments in triplicate, and presented as mean ± SEM, n = 3. Symbol denotes significance, *P < 0.05 versus corresponding control