Fig. 2
From: Vitamin E succinate inhibits survivin and induces apoptosis in pancreatic cancer cells
VES induces apoptosis in COLO-357 cells. a The morphology of COLO-357 cells treated with 40 and 80 μM VES after 24 h; cell shrinkage, blebs appearance. Original magnifications, 400 ×. b and c The expression of apoptotic indicators, cleaved PARP and cleaved caspase 3, in COLO-357 was determined by immunoblotting; β actin was used as loading control. Cells were treated at various concentrations of VES (10, 20, 40, and 80 μM) after 24 h. d DNA fragmentation was also examined at 40 and 80 μM VES treatment for 24 h. e Apoptosis was determined by the flow cytometric analysis of cells treated with VES at various concentrations (10, 20, 40, 60, and 80 μM) for 24 h. The percentage of cells containing sub-G1 DNA content was used as an index of apoptosis. P < 0.05 was considered significant. Asterisk denotes P < 0.01 as compared to control