Table 1 Primers used for RTPCR analysis of GPX1, SELW, SELH, GPX4, GPX2, GAPDH and IL8 transcripts
Primers | Sequences | Tm |
|---|---|---|
GPX4 for | 5′-CGA TAC GCT GAG TGT GGT TTG C-3′ | 66°C |
GPX4 rev | 5′-CAT TTC CCA GGA TGC CCT TG-3′ | 29cyc |
GAPDH for | 5′-TGA AGG TCG GAG TCA ACG GAT TTG-3′ | 55°C |
GAPDH rev | 5′-CAT GTA AAC CAT GTA GTT GAG GTC-3′ | 29cyc |
GPX1 for | 5′-CAG TCG GTG TAT GCC TTC TCG-3′ | 56°C |
GPX1 rev | 5′-TGT CAG GCT CGA TGT CAA TG-3′ | 27cyc |
GPX2 for | 5′-GGC TTT CAT TGC CAA GTC CTT C-3′ | 60°C |
GPX2 rev | 5′-CTA TAT GGC AAC TTT AAG GAG GCG C-3′ | 27cyc |
SELW for | 5′-GTT TAT TGT GGC GCT TGA GGC-3′ | 60°C |
SELW rev | 5′-GAA CAT CAG GGA AAG ACC ACC-3′ | 27cyc |
SELH for | 5′-GCT TCC AGT AAA GGT GAA CCC G-3′ | 62°C |
SELH rev | 5′-ACC CAA ATC TCC CTA CGA CAG G-3′ | 27cyc |
IL8 for | 5′-ATG ACT TCC AAG CTG GCC GTG GCT-3′ | 60°C |
IL8 rev | 5′-TCT CAG CCC TCT TCA AAA ACT TCT C-3′ | 31cyc |