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Fig. 1 | Genes & Nutrition

Fig. 1

From: Growth factor TGF-β induces intestinal epithelial cell (IEC-6) differentiation: miR-146b as a regulatory component in the negative feedback loop

Fig. 1

TGF-β induces IEC-6 cell differentiation. IEC-6 cells in 6-well plates were treated with 50 ng/ml TGF-β, and the control group was grown in growth media alone. After 48 h treatment, cell morphology and mRNA expression of intestinal epithelial cell markers were analyzed by H&E staining and real-time Q-PCR, respectively. a Cell morphology. Treated cells are distributed more evenly, contain more abundant cytoplasm, and have larger size and smaller numbers of nucleoli. Cells also showed intercellular connections around the cytoplasm and well-delineated cell borders, indicating epithelial cell morphology. Cells in the control group tended to aggregate and contained sparse plasma, small but many nucleoli that are related to the active division ability; b Gene expression. TGF-β significantly up-regulated the mRNAs for alkaline phosphatase, villin, and aminopeptidase N (P < 0.05). Values are means ± SEM run in triplicates; asterisks indicate significant differences between TGF-β treatment and control groups

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