Dietary supplementation with methyl donors reduces fatty liver and modifies the fatty acid synthase DNA methylation profile in rats fed an obesogenic diet

Table 1 Weight-related and plasma measurements of male Wistar rats after 8 weeks of dietary treatments

	Male dietary groups
	C (n = 7)	HFS (n = 9)	HFSsupp (n = 8)	ANOVA
Weight-related measurements
Weight gain (g)	152.6 ± 7.3a	191.8 ± 13.0b	198.8 ± 14.0b	*
Subcutaneous WAT (%)	1.89 ± 0.09a	3.14 ± 0.19b	3.10 ± 0.13b	***
Retroperitoneal WAT (%)	2.83 ± 0.25a	3.65 ± 0.16b	3.72 ± 0.21b	*
Epididymal WAT (%)	2.32 ± 0.20a	3.10 ± 0.11b	3.32 ± 0.20b	**
Mesenteric WAT (%)	1.06 ± 0.11a	1.45 ± 0.09b	1.45 ± 0.13b	*
Liver (%)	2.32 ± 0.03a	2.37 ± 0.06a	2.25 ± 0.05a	ns
Gastrocnemius muscle (%)	0.54 ± 0.02a	0.51 ± 0.01a	0.51 ± 0.01a	ns
Plasma values
Glucose (mg/dL)	114.5 ± 5.3a	134.0 ± 4.7b	122.9 ± 5.4a,b	*
HDL cholesterol (mg/dL)	69.2 ± 3.8a	72.0 ± 2.8a	79.6 ± 3.0a	ns
Triglycerides (mg/dL)	108.4 ± 8.8a	100.0 ± 11.3a	103.1 ± 9.6a	ns
Lactate (mg/dL)	25.6 ± 3.2a,b	32.8 ± 3.2a	22.1 ± 1.1b	*
Homocysteine (μmol/L)	17.9 ± 1.0a	18.7 ± 3.1a	14.3 ± 1.0a	ns
Insulin (μU/mL)	20.3 ± 2.4a	43.1 ± 5.0b	46.7 ± 3.6b	***

All the results are expressed as the mean ± SE. Statistical analyses were performed using one-way Anova and Tukey post hoc test. Different letters (a, b) indicate significant differences between groups of at least p < 0.05
WAT white adipose tissue, C control group, HFS high-fat-sucrose group, HFSsupp high-fat-sucrose supplemented with methyl donors group, ns non statistically significant
* p < 0.05, ** p < 0.01, *** p < 0.001

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