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Fig. 1 | Genes & Nutrition

Fig. 1

From: The soybean peptide lunasin promotes apoptosis of mammary epithelial cells via induction of tumor suppressor PTEN: similarities and distinct actions from soy isoflavone genistein

Fig. 1

SPI sera promote apoptosis and nuclear PTEN localization in MCF-7 cells. a Dual immunofluorescent staining of MCF-7 cells treated with sera from CAS or SPI-fed adult rats and visualized using fluorescein-labeled TdT reagent for apoptotic cells (green) and rhodamine (red) for PTEN-positive cells prior to counterstaining with 4′,6-diamidino-2-phenylindole (DAPI; blue). Co-localization of TUNEL (apoptotic cells; T) and PTEN-stained cells (P) coincide with DAPI (D + T + P), the latter shown as purple color. Representative images for each group are depicted at 400× magnification from three independent experiments; Bar 100 μm. (b–d). The percentages of TUNEL and nuclear PTEN-positive cells were calculated by counting at least 500 cells from five random areas per slide with at least three slides per treatment group. The densitometric means for nuclear PTEN-positive cells were quantified using Axiovision software. Means (±SEM) with asterisk are significantly different (P < 0.05, Student’s t test) from the control CAS group

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