Fig. 3

Lunasin promotion of MCF-7 cell apoptosis is mediated by PTEN. a Representative images of dual immunofluorescent-stained MCF-7 cells treated with vehicle or Lunasin (LUN; 2 μM). Stained cells indicate: PTEN-positive (P, Red), Apoptotic (T, green), and nuclear compartment (D; blue). Nuclear co-localization of PTEN-(P) and TUNEL (T)-positive cells is shown as D + P + T; Bar, 50 μm. b Dose-dependent induction of apoptosis of MCF-7 cells by LUN is shown as percent TUNEL-positive cells. c MCF-7 cell apoptotic status after treatment for 24 h with LUN (50 nM and 2 μM) in the presence of PTEN siRNAs or non-targeting (control) siRNAs. The percent of TUNEL-positive cells was determined by counting five random areas per slide and at least three slides per treatment group. Means (±SEM) with different superscripts are significantly different (P < 0.05, one-way ANOVA)