Fig. 4

Lunasin effects on MCF-7 cells are not p53-mediated. a Nuclear extracts isolated from MCF-7 cells treated with vehicle or 2 μM Lunasin (LUN) for 24 h were analyzed by western blotting for p53 protein. Lamin A was used as loading control. A representative western blot is shown, with each lane representing samples from an independent experiment. Bar graph represents densitometric scan of p53 bands normalized to Lamin A. Means values (±SEM) are not different (P > 0.05, Student’s t test). b Gene expression levels of p53, C-MYC, and CCND1 in MCF-7 cells treated with vehicle or 2 μM LUN for 24 h were analyzed by qRT-PCR. Transcript levels were normalized with 18S and renormalized to vehicle group. Means values (±SEM) are not different (P > 0.05, Student’s t test)