Fig. 4
Western blot analysis of folate transporters in liver. Western blots analysis of liver tissue lysate (a) using anti-RFC (58 kDa), anti-PCFT (54 kDa), and anti FBP (27 kDa) antibodies with β-actin (43 kDa) as an internal control (b) Graph represents summary data of densitometric analysis, lane M- marker, lane 1, 2: control; 3, 4: ethanol fed. Western blot analysis of the liver BLM (c) using anti-RFC (58 kDa), anti-PCFT (54 kDa) antibodies. d Graph represents summary data of densitometric analysis. Data are expressed are mean ± SD of four separate experiments. Lanes: 1, control; 2, ethanol fed. **p < 0.01; ***p < 0.001 versus control