Fig. 3

Effects of RSV treatment on high-glucose-induced VEGF levels. Confluent cells were treated with different doses of RSV for 72 h, respectively. The supernatant of cell culture medium was collected for ELISA testing of secreted VEGF. (a) The mRNA level of VEGF after incubation. Comparative Ct method with GADPH as reference was adopted. (b) The secreted amount of VEGF in supernatant of cell medium, the unit is ng/L. (*P < 0.05 vs. the control; ^# P < 0.05 vs. the HG group). Data are expressed as the mean ± SEM (N = 6)