Time-dependent network analysis reveals molecular targets underlying the development of diet-induced obesity and non-alcoholic steatohepatitis

Table 1 Phenotype biomarkers of high-fat-fed mice

Biomarkers	0 week	24 weeks
Biomarkers	Baseline	ND	HFD
Body weight gain (g)	18.02 ± 0.39	33.43 ± 0.86	45.92 ± 0.60***
Total WAT (g/100 g B.W.)	1.70 ± 0.09	13.04 ± 0.72	22.55 ± 0.42***
Liver weight (g/100 g B.W.)	4.32 ± 0.06	3.78 ± 0.11	6.97 ± 0.18***
Fasting blood glucose (mg/dL)	80.83 ± 4.69	114.38 ± 5.39	133.00 ± 2.98**
Hepatic cholesterol (mmol/g Liver)	0.48 ± 0.03	2.63 ± 0.28	9.07 ± 1.07***
Hepatic FFA (mmol/g Liver)	1.35 ± 0.16	2.55 ± 0.20	7.43 ± 0.46***
Hepatic triglycerides (mg/g Liver)	6.27 ± 0.49	19.49 ± 1.20	37.70 ± 2.67***
Plasma total cholesterol (mmol/L)	1.70 ± 0.06	4.38 ± 0.13	10.02 ± 0.28***
Plasma LDL-C (mmol/L)	1.20 ± 0.01	3.37 ± 0.16	8.27 ± 0.27***
Plasma HDL-C (mmol/L)	0.24 ± 0.005	0.91 ± 0.04	1.62 ± 0.06***
Atherogenic index (A.U)	5.83 ± 0.07	3.85 ± 0.25	5.95 ± 0.33***

Data are Mean ± SE
ND normal diet (AIN-76), HFD high-fat diet (20 % fat, 1 % cholesterol), BW body weight, FFA free fatty acid, Total WAT total white adipose tissue = epididymal fat + Perirenal fat + Retro-peritoneum fat + Mesentery fat + Subcutaneous fat + Interscapular WAT
Values are significantly different from the ND group according to Student’s t test: * p < 0.05; ** p < 0.01; *** p < 0.001

ISSN: 1865-3499