Hepatic and plasma sex differences in saturated and monounsaturated fatty acids are associated with differences in expression of elongase 6, but not stearoyl-CoA desaturase in Sprague–Dawley rats

Table 2 Phospholipid fatty acid composition of HepG2 cells incubated with varying supplemented concentrations of 17β-estradiol for 72 h

Fatty acid	μg fatty acid/mg cellular protein
Fatty acid	0 nM	10 nM	30 nM	100 nM
12:0	0.12 ± 0.05	0.18 ± 0.07	0.11 ± 0.03	0.15 ± 0.08
14:0	0.86 ± 0.15	1.00 ± 0.08	0.87 ± 0.17	0.91 ± 0.18
16:0	12 ± 1^ab	15 ± 1^a	11 ± 2^b	12 ± 2^ab
18:0	5 ± 1	6 ± 1	5 ± 2	5 ± 1
20:0	0.10 ± 0.02	0.13 ± 0.02	0.13 ± 0.04	0.12 ± 0.01
22:0	0.16 ± 0.02	0.16 ± 0.05	0.15 ± 0.03	0.15 ± 0.04
23:0	0.12 ± 0.01	0.13 ± 0.04	0.12 ± 0.03	0.11 ± 0.02
24:0	0.08 ± 0.01	0.05 ± 0.05	0.07 ± 0.01	0.07 ± 0.02
Total SFA	18 ± 2	22 ± 2	18 ± 4	18 ± 2
14:1	0.19 ± 0.03^ab	0.25 ± 0.02^a	0.17 ± 0.02^b	0.21 ± 0.04^ab
16:1n-7	3.68 ± 0.42^ab	4.63 ± 0.35^a	3.50 ± 0.61^b	3.70 ± 0.57^ab
18:1n-7	6.33 ± 0.57^a	7.87 ± 0.60^b	5.83 ± 0.86^a	6.19 ± 0.92^a
18:1n-9	8.34 ± 0.72^a	10.46 ± 0.63^b	7.60 ± 0.98^a	8.18 ± 1.43^a
20:1n-9	0.18 ± 0.03	0.21 ± 0.04	0.16 ± 0.04	0.16 ± 0.02
22:1n-9	0.27 ± 0.20	0.34 ± 0.20	0.22 ± 0.20	0.36 ± 0.28
24:1n-9	0.18 ± 0.01	0.19 ± 0.05	0.17 ± 0.04	0.17 ± 0.06
Total MUFA	19 ± 2^a	24 ± 1^b	18 ± 2^a	19 ± 3^a
Total n-6 PUFA	3.29 ± 0.37^ab	3.96 ± 0.36^a	3.03 ± 0.43^b	3.17 ± 0.53^ab
Total n-3 PUFA	1.4 ± 0.17^ab	1.67 ± 0.13^a	1.28 ± 0.20^b	1.33 ± 0.21^ab
Total fatty acids	48.53 ± 4.85^ab	59.33 ± 3.65^a	45.36 ± 7.70^b	47.78 ± 7.58^ab

Values are mean ± standard deviation, n = 5 per group
Values with different superscripts are significantly different by Tukey’s post hoc test (p < 0.05) following significant F value by one-way ANOVA

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