Differences in metabolomic and transcriptomic profiles between responders and non-responders to an n-3 polyunsaturated fatty acids (PUFAs) supplementation

Table 3 Erythrocytes fatty acid profiles of responders and non-responders pre- and post-n-3 PUFA supplementation

(% of free FA)	Responders (n = 6)			Non-responders (n = 6)			P value between resp. and non-resp.
(% of free FA)	Pre-n-3 PUFAs	Post-n-3 PUFAs	P value	Pre-n-3 PUFAs	Post-n-3 PUFAs	P value	Pre-n-3 PUFAs	Delta (post- and pre-n-3 PUFAs)
Stearic acid (18:0)	15.37 ± 0.33	15.42 ± 0.27	NS	15.03 ± 0.34	15.10 ± 0.33	NS	NS	NS
Oleic acid (18:1n-9)	11.93 ± 0.10	11.56 ± 0.25	NS	11.57 ± 0.29	11.46 ± 0.32	NS	NS	NS
Arachidonic acid (20:4n-6)	14.06 ± 0.16	12.87 ± 0.28	0.0118	14.10 ± 0.27	12.87 ± 0.19	0.0004	NS	NS
Dihomo-gamma-linolenic acid (20:3n-6)	2.06 ± 0.26	1.56 ± 0.19	0.0051	1.83 ± 0.07	1.43 ± 0.10	0.0054	NS	NS
Eicosapentanoic acid (20:5n-3)	0.67 ± 0.09	2.33 ± 0.23	<0.0001	0.77 ± 0.12	2.12 ± 0.24	0.0044	NS	NS
Docosahexaenoic acid (22:6n-3)	4.01 ± 0.56	5.05 ± 0.43	0.0059	4.10 ± 0.35	5.36 ± 0.47	NS	NS	NS
Total N-6	29.10 ± 0.71	25.54 ± 0.92	0.0002	29.44 ± 0.48	25.99 ± 0.57	0.0010	NS	NS
Total N-3	7.11 ± 0.66	10.38 ± 0.71	<0.0001	7.00 ± 0.52	10.21 ± 0.73	0.0014	NS	NS
Ratio total N-3/total N-6	0.25 ± 0.03	0.41 ± 0.04	0.0001	0.24 ± 0.02	0.40 ± 0.04	0.0018	NS	NS
Desaturase indices								NS
SCD18 (18:1n-9/18:0)	0.78 ± 0.02	0.75 ± 0.02	0.0153	0.77 ± 0.02	0.76 ± 0.02	NS	NS	NS
FADS1 D5D (20:4n-6/20:3n-6)	7.31 ± 0.78	8.80 ± 0.95	0.0053	7.76 ± 0.29	9.17 ± 0.61	0.0174	NS	NS
ELOVL2 (22:6n-3/20:5n-3)	6.04 ± 0.32	2.20 ± 0.10	<0.0001	5.60 ± 0.48	2.67 ± 0.37	0.0057	NS	NS

Data are shown as mean ± SEM; supplementation is the effect of n-3 PUFA supplementation on parameters
RBC red blood cells, FA fatty acid, EPA eicosapentanoic acid, DHA docosahexaenoic acid

ISSN: 1865-3499