Fig. 2

Broccoli does not affect food intake. Beetles were starved for 6 h before they were fed on flour alone or supplemented with 0.1 or 10 % broccoli or in the absence of food (control) either at 32 °C (a) or 42 °C (b). After 2, 4 or 6 h individuals were frozen at −18 °C, and food intake was determined by measuring the wet weight of 10 pooled individuals per setting on a microbalance with 8 replicates per broccoli concentration and time point. ***p < 0.001 for beetles fed on flour ± broccoli versus beetles in the absence of food