Fig. 5

Microinjection of dsRNA into T. castaneum pupae induces efficient RNAi. GFP fluorescence in the T. castaneum reporter strain was visualized by fluorescence microscopy, with wings folded to reveal muscle fluorescence. Beetles were left untreated (a) or treated with dsRNA to knock down GFP either by feeding them with living RNAi bacteria (b), lyophilized RNAi bacteria (c), by feeding in vitro transcribed dsRNA (d) or by microinjecting in vitro transcribed dsRNA into the imagos (e) or the pupae (f). Images were captured 72 h after the treatments