Table 1 Sequences of the primers used for RT-qPCR
Genes | Primer sequence (5′–3′) | Annealing temperature (°C) | Amplicon size (bp) | References |
|---|---|---|---|---|
TNF-α Sae F TNF-α Sae R | agc cca tgt tgt agc aaa cc tga ggt aca ggc cct ctg at | 58 | 134 | Saegusa et al. (2007) |
IL-6 Tatjana F IL-6 Tatjana R | ttc aat gag gag act tgc ctg aca aca aca atc tga ggt gcc | 60 | 349 | Faruqi et al. (2001) |
β-Actin F β-Actin R | tgg ctg ggg tgt tga agg tct agc acg gca tcg tca cca act | 58 | 238 | Lallemant et al. (2009) |
TNF-α mus F TNF-α mus R | tct cat cag ttc tat ggc cc ggg act aga caa ggt aca ac | 54 | 212 | Dombrowicz et al. (2001) |
IL-6 mus F IL-6 mus R | gtt ctc tgg gaa atc gtg ga tgt act cca ggt agc tat gg | 54 | 209 | Dombrowicz et al. (2001) |
MCP-1 mus F MCP-1 mus R | tct ggg cct gct gtt cac gtg aat gag tag cag cag gtg agt | 54 | 96 | This study |
VCAM-1MF1 VCAM-1MR1 | tgg tga aat gga atc tga acc ccc aga tgg tgg ttt cct t | 54 | 86 | This study |
ICAM-1MF1 ICAM-1MR1 | gga cca cgg agc caa ttt c ctc gga gac att aga gaa caa tgc | 55 | 82 | This study |
E-selectin MF E-selectin MR | ccc tgc cca cgg tat cag ccc ttc cac aca gtc aaa cgt | 55 | 85 | This study |
ACTB mus2F ACTB mus2R | agt gtg acg ttg aca tcc gta gcc aga gca gta atc tcc ttc t | 54 | 112 | This study |