Table 1 Primers and the annealing temperatures used for studying the gene expression in rat
Gene | Primer 5′–3′ | Tm (°C) | References |
|---|---|---|---|
Leptin | f-GAGACCTCCTCCATCTGCTG r-CTCAGAGCCACCACCTCTGT | 58.9 | |
LEPR | f-GAGAGGCTGCTGAAATCGTC r-CTCCAGACTCCTGAGCCATC | 58.9 | |
LEPR-a | f-CACTGTTAATTTCACACCAGAG r-GTCATTCAAACCATAGTTTAGG | 58.9 | (da Silveira Cavalcante et al. 2009) |
LEPR-b | f-GCATGCAGAATCAGTGATATTTGG r-CAAGCTGTATCGACACTGATTTCTTC | 61.4 | (Lukaszewski et al. 2011) |
LEPR-c | f-TGCTCGGAACACTGTTAAT r-ATAGAGTATCTAACCTGCACCCTT | 58.9 | (da Silveira Cavalcante et al. 2009) |
LEPR-e | f-TCGTGGACACTGTCACCTAA r-ATCAGGATTGCCAATTTACA | 58.9 | (da Silveira Cavalcante et al. 2009) |
LEPR-f | f-GCTGCTCGGAACACTGTTAAT r-ACGGCATCCACTCTATATCCT | 58.9 | (da Silveira Cavalcante et al. 2009) |
SOCS3 | f-GCCAGTGCCCCGCTTTGACT r-GAGGAGGGTTCCGTCGGTGGT | 64.5 | |
ACC | f-GCCTCTTCCTGACAAACGAG r-TCCATACGCCTGAAACATGA | 61.4 | |
ATG7 | f-GCTGGTCTCCTTGCTCAAAC r-CAGGGTGCTGGGTTAGGTTA | 63.3 | |
MAP1LC3β | f-CGTCCTGGACAAGACCAAGT r-AGTGCTGTCCCGAACGTCTC | 61.4 | (Sengupta et al. 2009) |
LAMP2 | f-AGCACTATTGGGGCATTCAC r-GGTGTGAATGATGGGTGCTA | 61.4 | |
GAPDH | f-ATGACTCTACCCACGGCAAG r-GGAAGATGGTGATGGGTTTC | 58.9 | |
18S rRNA | f-AGTCGGCATCGTTTATGGTC r-CGCGGTTCTATTTTGTTGGT | 58.9 | |
HPRT | f-TCCCAGCGTCGTGATTAGTGA r-CCTTCATGACATCTCGAGCAAG | 58.9 | (Martinez-Beamonte et al. 2011) |
UBC | f-ATCTAGAAAGAGCCCTTCTTGTGC r-ACACCTCCCCATCAAACCC | 58.9 | (Martinez-Beamonte et al. 2011) |