Exposure of neonatal rats to maternal cafeteria feeding during suckling alters hepatic gene expression and DNA methylation in the insulin signalling pathway

Table 2 DNA Methylation in liver of 3-month-old rats

	Dietary exposure groups
	CC		CO		OC		OO
	Male	Female	Male	Female	Male	Female	Male	Female
Global methylation (pg MDC/μg DNA)	190 ± 11	174 ± 12	154 ± 4	176 ± 4	166 ± 12	160 ± 6	120 ± 11	212 ± 5
Methylation PPARg (%)	0.47 ± 0.17	0.42 ± 0.20	0.61 ± 0.38	0.48 ± 0.23	9.24 ± 6.63	9.19 ± 6.62	0.27 ± 0.04	0.21 ± 0.04
Methylation IrB (%)	0.60 ± 0.14	0.54 ± 0.12	1.43 ± 0.68	0.91 ± 0.68	13.1 ± 7.72	13.1 ± 7.68	0.46 ± 0.05	0.49 ± 0.02

Data are shown as mean ± SEM for 5–6 observations per group. Global methylation was influenced by the interaction of sex and post-weaning diet (P = 0.018). Methylation of PPARg was not influenced by sex or diet at any stage. IrB methylation was significantly influenced by the interaction of diet in lactation and weaning (P = 0.036), with univariate influences of lactation (P = 0.058) and post-weaning (P = 0.054) diets approaching significance. MDC-5-methyl-2′-deoxy cytidine

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