Conjugated and free sterols from black bean (Phaseolus vulgaris L.) seed coats as cholesterol micelle disruptors and their effect on lipid metabolism and cholesterol transport in rat primary hepatocytes

Table 1 Free phytosterols, steryl glycosides, and acylated steryl glycosides found in black bean seed coat based on accurate mass and comparison with literature

Compound	Molecular mass	m/z	Positive MS assignment	References
Free sterols
Stigmasterol	412	395.36	[M + H − H₂O]⁺	Ongoka et al. (2008), Rozenberg (2003)
Campesterol	400	383.35	[M + H − H₂O]⁺	Rozenberg (2003)
		411.35	[M + H − 2H]⁺	Rozenberg (2003)
		821.69	[M + M − H]⁺
β-Sitosterol	414	397.37	[M + H − H₂O]⁺	Kishore et al. (2011), Rozenberg (2003)
Δ⁵-Avenasterol	412	395.35	[M + H − H₂O]⁺	Rozenberg (2003)
		413.26	[M + H]⁺
Steryl glycosides
Δ⁵-Avenasterol-β-d-glucoside	574	395.27	[Aglycone − 3H − H₂O]⁺	Rozenberg (2003)
		413.26	[Aglycone + H]⁺
		592.48	[M + H₂O]
Campesteryl-β-d-glucoside	562	383.35	[Aglycone + H − H₂O]⁺
		419.25	[Aglycone + H₂O + H]⁺	Dweck (2006), Rozenberg (2003)
		579.28	[M + H₂O − H]⁺
		595.25	[M + H₂O + 3H]⁺
Acylated steryl glycosides
Campesteryl (6′-O-linoleoyl)-β-d-glucoside	824	383.36	[Aglycone + H − H₂O]⁺	Leisso et al. (2013)
		821.69	[M + H − 4H]⁺	Rozenberg (2003)
β-Sitoesteryl(6′-O-linoleoyl)-β-d-glucoside	838	837.56	[M + H − 2H]⁺	Rozenberg (2003)
		397.37	[Aglycone + H − H₂O]⁺	Rozenberg (2003)

ISSN: 1865-3499