Fig. 3
From: Reduced expression of folate transporters in kidney of a rat model of folate oversupplementation
[14C]-5-methyltetrahydrofolate transport in renal BBMV at various a time and b pH points. The incubation buffer [100 mM NaCl, 80 mM mannitol, 10 mM HEPES, 10 mM 2-morpholinoethanesulfonic acid (MES)] containing 0.5 μM [14C]-5 methyltetrahydrofolate was used for uptake measurements. a Transport was studied at different time intervals at a constant pH of 5.5. b Uptake was measured by varying incubation buffer pH from 5.0 to 8.0, keeping intravesicular pH 7.4. Each data point is mean ± SD of 4 separate uptake determinations carried out in duplicate. *p < 0.05; **p < 0.01; ***p < 0.001 versus control