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Fig. 3 | Genes & Nutrition

Fig. 3

From: Coffee intake mitigated inflammation and obesity-induced insulin resistance in skeletal muscle of high-fat diet-induced obese mice

Fig. 3

Changes in glucose levels and insulin signaling during insulin stimulation. a Glucose levels and AUCglucose during the ITT. At 150 min after the insulin administration, there was a significant decrease in glucose levels in the HFGC mice compared to the HF mice, which is evident from the significantly lower AUCglucose values in the HFGC group. The glucose concentrations and AUCglucose values of the HFCC and HFDC groups also tended to be lower than that of the HF group. b Levels of tyrosine phosphorylated IRS-1, p85/IRS-1, and Ser473 phosphorylated Akt/PKB. The bands of immunoreactive proteins were detected with an enhanced chemiluminescence kit and quantified using an LAS-1000-plus image analyzer. Insulin administration markedly elevated the tyrosine phosphorylation of IRS-1, the levels of p85/IRS-1 complex, and the serine473 phosphorylation of Akt/PKB, most of which were suppressed in the HF group. However, the tyrosine phosphorylation levels of IRS-1 tended to increase in all coffee groups compared to the HF group. The levels of the p85/IRS-1 complex were improved in the HFCC and HFGC mice, and the serine473 phosphorylated Akt/PKB levels also tended to higher in the HFCC group. All data are presented as mean ± SE (ND, HF: n = 4; HFCC, HFDC, HFGC: n = 5) of the fold changes normalized to the levels of each sample to that of the ND group. *p < 0.05; **p < 0.01 versus the HF group by Dunnett’s test

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