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Table 1 Effect of vitamin C supplementation on gene expression level in PBMNC, assessed by means of Affymetrix and qPCR platforms

From: Vitamin C supplementation modulates gene expression in peripheral blood mononuclear cells specifically upon an inflammatory stimulus: a pilot study in healthy subjects

Official gene symbol

Gene name

Affymetrix

Representative pathwaysc

QPCR

FCa

p valueb

FCd

p valuee

CANX

Calnexin

1.13

0.0014

Chromatin organization

  

CANX(1)

Calnexin1

  

Chromatin organization

0.18 ± 0.12

N.S.f

CANX(2)

Calnexin2

  

Chromatin organization

0.53 ± 0.37

0.021

ARF3

ADP-ribosylation factor 3

0.98

0.0003

Chromatin organization

  

SMNDC1

Survival motor neuron domain containing 1

0.95

0.0054

Chromatin organization

  

RPS24

Ribosomal proteinS24

0.89

0.0015

Chromatin organization

  

MYST2

Histone acetyltransferase MYST2

0.87

0.0320

Chromatin organization; ribonucleoprotein complex biosynthesis

  

NONO

Non-POU domain containing

0.81

0.0040

Ribonucleoprotein complex biosynthesis; RNA processing

  

ZNF259

Zinc finger protein 259

0.81

0.0017

Ribonucleoprotein complex biosynthesis; RNA processing

  

FNTA

Farnesyl-protein transferase alpha subunit

0.79

0.0041

Ribonucleoprotein complex biosynthesis; translation; RNA processing

  

Y_RNA

Small noncoding RNA components of the Ro ribonucleoprotein

0.77

0.0101

Ribonucleoprotein complex biosynthesis; translation; RNA processing

  

HRH4

Histamine receptor H4

0.74

0.0191

Ribonucleoprotein complex biosynthesis; translation; RNA processing

  

IK

IK cytokine

0.73

0.0234

Ribonucleoprotein complex synthesis

0.15 ± 0.44

N.S.

CTLA4

Cytotoxic T-lymphocyte antigen 4

0.7

0.0234

RNA processing

  

U1

 

0.68

0.0045

RNA processing

  

5S_rRNA

RRNA

0.68

0.0325

translation

  

EIF3D

Eukaryotic translation initiation factor 3, subunit D

0.66

0.0018

Translation

0.008 ± 0.43

N.S.

EIF4G2

Eukaryotic translation initiation factor 4 gamma

0.66

0.0085

Translation

0.24 ± 0.19

N.S.

FAU

Finkel–Biskis–Reillymurine sarcoma virus (FBR-MuSV)

0.66

0.0310

 

0.66 ± 0.16

0.008

SART3

Squamous cell carcinoma antigen recognized by T cell 3

0.64

0.0140

   

AC068228.1

tRNA pseudogene

0.63

0.0016

   

RPL11

Ribosomal protein L11

0.62

0.0045

   

C11orf58

Small acidic protein

0.61

0.0234

   

C1D

Nuclear receptor co-repressor

0.6

0.0288

   

HINT1

Adenosine 5′-monophosphoramidase

0.57

0.0212

   

CBX3

Heterochromatin protein 1 homolog gamma

0.54

0.0129

   

NPM1

Nucleophosmin 1

0.53

0.0090

 

0.25 ± 0.75

N.S.

RPS7

40S Ribosomal protein S7

0.52

0.0186

   

HIST1H2BB

Histone H2B type 1-B

−0.57

0.0254

   

SNORA42

Small nucleolar RNA

−0.65

0.0069

   

RNU5E

RNA, U5E small nuclear

−0.86

0.0116

   

AC025562.1

ScRNA pseudogene

−0.55

0.0210

   

AL139097.1

RRNA pseudogene

−0.52

0.0086

   

AL138832.1

RRNA pseudogene

−0.61

0.0023

   

AP003461.5

Mt tRNA pseudogene

−0.51

0.0036

   

AC105285.2

ScRNA pseudogene

−0.56

0.0297

   

AL358813.3

tRNA pseudogene

−0.84

0.0064

   

AL953889.1

ScRNA pseudogene

0.52

0.0356

   

RP11613C6.4

Processed pseudogene

0.75

0.0390

   

AL353774.1

tRNA pseudogene

0.55

0.0408

   
  1. aData are expressed by mean of log2 fold change (FC). FC threshold cutoff ≤ ≥ 0.5
  2. bStatistical significance of differences in microarray expression between supplemented PBMNC and baseline PBMNC, estimated by ANOVA test (p < 0.05)
  3. cThe overrepresented biological pathway was identified by DAVID functional annotation clustering tool
  4. dData are expressed as mean ± SD of log2 of fold change (FC)
  5. eStatistical significance of differences in gene expression between supplemented PBMNC and baseline PBMNC, estimated by paired Student’s t test (p < 0.05; threshold cutoff ≤ ≥ 0.5)
  6. fN.S. no significant difference