Table 1 Primer sequences of target and reference genes as well as their biological function
From: Immunomodulatory activity of two potential probiotic strains in LPS-stimulated HT-29 cells
Name | Forward primer (5′–3′) | Reverse primer (5′–3′) | Product size (bp) | Tm (°C) | References |
|---|---|---|---|---|---|
ALAS1 | aac ttg cca aaa tct gtt tc | ggt gat gag gga gtc tga at | 159 | 81.47 | Lallemant et al. (2009) |
B2Â M | cag cgt act cca aag att ca | gaa tgc tcc act ttt tca at | 240 | 81.45 | Lallemant et al. (2009) |
HMBS | gaa aga caa cag cat cat gag | acc aag gag ctt gaa cat gc | 145 | 85.56 | Lallemant et al. (2009) |
HPRT1 | ctg acc tgc tgg att aca | gcg acc ttg acc atc ttt | 256 | 81.77 | Lallemant et al. (2009) |
RPL27 | tcg cca aga gat caa aga taa | ctg aag aca tcc tta ttg acg | 121 | 80.04 | Lallemant et al. (2009) |
RPS18 | agc ttg ttg tcc aga cca tt | tga gga aag cag aca ttg ac | 187 | 84.28 | Lallemant et al. (2009) |
SDHA | agc aag ctc tat gga gac ct | taa tcg tac tca tca atc cg | 200 | 88.68 | Lallemant et al. (2009) |
TBP | cac gaa cca cgg cac tga tt | ttt tct tgc tgc cag tct gga c | 89 | 82.74 | Lallemant et al. (2009) |
ACTB | tgg ctg ggg tgt tga agg tct | agc acg gca tcg tca cca act | 238 | 88.62 | Lallemant et al. (2009) |
GAPDH | caa cga cca ctt tgt caa gc | ttc ctc ttg tgc tct tgc tg | 140 | 85.88 | This study |
MUC2 | cag cac cga ttg ctg agt tg | gct ggt cat ctc aat ggc ag | 140 | 86.5 | Hatayama et al. (2007) |
IL12p35 | ttc acc act ccc aaa acc tgc | gag gcc agg caa ctc cca tta | 225 | 84.5 | Maaser et al. (2004) |
IL-8 | tgg ctc tct tgg cag cct tc | tgc acc cag ttt tcc ttg gg | 238 | 83.0 | Saegusa et al. (2007) |
Cox1 | gca acc tca aca cca cct tc | ccg aag cct ggt agg ata ag | 118 | 82.0 | This study |
Cox2 | caa ttc ccg gac gtc taa acc | cta gga cga tgg gca tga aac | 114 | 83.5 | This study |
IL-10 | aaa gaa ggc atg cac agc tc | aag cat gtt agg cag gtt gc | 132 | 90.0 | This study |
TNF-α | agc cca tgt tgt agc aaa cc | tga ggt aca ggc cct ctg at | 134 | 89.5 | Saegusa et al. (2007) |
hsp70 | gat cat ggg ttg ctt ctt cc | gaa gcc aaa tac tgc cat cc | 128 | 83.5 | This study |
IFN- α | ctg aaa cca tcc ctg tcc tc | cac agg ctt cca ggt cat tc | 147 | 83.5 | This study |
TGF-β2 | atg ccc gta ttt atg gag tt | att gtc att ttg gtc ttg cc | 234 | 83.0 | Saegusa et al. (2007) |
IFN-γ | atc cag tta ctg ccg gtt tg | gaa gca cca ggc atg aaa tc | 135 | 82.0 | This study |