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Table 2 Efficacy of putative probiotic lactobacilli in inducing the relative expression of Cox-1 and Cox-2 genes in HT-29 cells under different experimental conditions

From: Immunomodulatory activity of two potential probiotic strains in LPS-stimulated HT-29 cells

Treatment

Fold change

MUC2

hsp70

Cox-1

Cox-2

Cox-1/Cox-2

Blank

0.24 ± 0.02

0.18 ± 0.01

0.40 ± 0.01

1.18 ± 0.05

0.34 ± 0.02

 LPS

0.37 ± 0.01

2.10 ± 0.13*

0.21 ± 0.02***

14.77 ± 0.63*

0.01 ± 0.001

 Lp9

1.49 ± 0.16*

0.97 ± 0.03*

0.61 ± 0.04***

0.20 ± 0.01

3.06 ± 0.35*

 Lp91

0.54 ± 0.01

0.73 ± 0.02*

1.26 ± 0.02*

0.75 ± 0.0

1.68 ± 0.03

 Lp5276

2.96 ± 0.19*

1.08 ± 0.05*

1.10 ± 0.07*

0.17 ± 0.00

6.47 ± 0.41*

Pre-treatment with probiotics prior to LPS stimulation

 Lp9

3.30 ± 0.16*

1.78 ± 0.07*

1.12 ± 0.06*

0.19 ± 0.01

5.92 ± 0.63*

 Lp91

3.70 ± 0.39*

1.08 ± 0.03*

1.04 ± 0.03*

0.16 ± 0.01

6.51 ± 0.22*

 Lp5276

4.33 ± 0.72*

1.36 ± 0.04*

0.77 ± 0.07*

0.07 ± 0.01

11.21 ± 2.43*

Co-culture treatment with probiotics and LPS

 Lp9

2.57 ± 0.08*

1.58 ± 0.12*

1.44 ± 0.15*

0.38 ± 0.01

3.78 ± 0.30*

 Lp91

1.26 ± 0.05**

0.76 ± 0.01*

1.83 ± 0.04*

0.42 ± 0.01

4.36 ± 0.01*

 Lp5276

5.59 ± 0.21*

1.98 ± 0.05*

1.15 ± 0.01*

0.25 ± 0.01

4.60 ± 0.14*

Post-probiotic treatment after LPS stimulation

 Post-Lp9

0.37 ± 0.05

1.09 ± 0.12*

0.20 ± 0.00***

17.87 ± 3.08*

0.01 ± 0.002

 Post-Lp91

0.43 ± 0.03

1.20 ± 0.18*

0.28 ± 0.04

15.98 ± 2.74*

0.02 ± 0.01

 Post-Lp5276

0.29 ± 0.01

1.52 ± 0.14*

0.16 ± 0.01**

14.05 ± 1.07*

0.01 ± 0.002

  1. Data are represented as mean ± SD; number of RT-qPCR experiments (n) = 3
  2. * P < 0.001 compared with blank; ** P < 0.01 compared with blank; *** P < 0.05 compared with blank