Table 1 PCR and pyrosequencing primer sequences
Genes | Analyzed zones and primer sequences | |
|---|---|---|
fasn | ||
−90 to −62 | Fwd Rev Seq | 5′-GTGTGGAAGTTAGATGATAATT-3′ 5′-BIO-CTTAAAACTCTAATCTTATAACCACCTTAC-3′ 5′-GTTAGATGATAATTTTTAAGTGGG-3′ |
+754 to +812 | Fwd Rev Seq | 5′-GGTTTGGGATTGGAAAGAGATT-3′ 5′-BIO-ACATATCTAAACTATATCCTTTTTACACCA-3′ 5′-GGATTGGAAAGAGATTGA-3′ |
+951 to +997 | Fwd Rev Seq | 5′-GAATTTTGGAAAATAAATATAGTGAGTGT-3′ 5′-BIO-ATAACCCTTAATCCCCAACACT-3′ 5′-ATTTTGGAAAATAAATATAGTGAG-3′ |
pnpla2 | ||
−205 to −162 | Fwd Rev Seq | 5′-GTAGGGTGTGGTGGAGAT-3′ 5′-BIO-TTCTACCCCTCCTACTACACT-3′ 5′-GGGTGTGGTGGAGAT-3′ |
+150 to +172 | Fwd Rev Seq | 5′-BIO-GGTGTTTAGATTTGTATAAAATTTG-3′ 5′-CACCTACTAAACAAAACCATCT-3′ 5′-TCTCAAACTTTAACTTCTCAT-3′ |
+349 to +391 | Fwd Rev Seq | 5′-GATGTTTTTAAGGGAGATTAAGT-3′ 5′-BIO-AACCACTCCAATATAATAAACC-3′ 5′-GATTAAGTGGAATATTT-3′ |
pparg | ||
−431 to −388 | Fwd Rev Seq | 5′-GGTTAGGAGGGTTATAGTGGAGTT-3′ 5′-BIO-AACTATCACCAAATCCACACAAT-3′ 5′-GGTTTTTTTAGAAGGTGTT-3′ |
−214 to −190 | Fwd Rev Seq | 5′-BIO-GGTGATAGTTTAAGTAATTTGGT-3′ 5′-ATAACCCCATTTTCCTCA-3′ 5′-CATTTTCCTCACACCTA-3 |